Research Area

1. Ultra-sensitive single-molecule diagnositics

Ultra-sensitive measurement of protein and nucliec acid is crucail for early disease diagnosis and disease prevention. We are endevour to develop ultrasensitive diagnositc systems with easy operability, high precision, rapidity and easy access.

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2. Functional nanobeads for biological labels

Assemblly of various functional motifs, such as quantum dots, gold nanoparticles, magnetic nanoparticles and aggregation induced fluorogens, into functional nanobeads were developled for biological labeling of antibody, nucleic acid and aptamers. The size, surface groups and intiror structures are studied to obtain the optimal labels for lateral flow immunoassays and digital assays.

photos by zpf.

主持完成国家自然科学基金，上海市自然科学基金，上海市生物医药科技支撑等项目多项

[15] Wenwen Xia#, Ying Wu#, Xiangyi Liao#, Lili Cui,Huanxing Han, Juanjuan Hou, Zhipeng Wang*, Pengfei Zhang*, ShouhongGao*, On-Site Therapeutic Drug Monitoring for Program Death-1 Antibody Using aQuantum Dot Nanobeads-Based Lateral Flow Immunoassay, Anal. Chem. 2025,27, 14612–14619.

[14] Xiaoyi Li, Wannian Yan, Yucheng Wang, Ru-jia Yu, HuanxingHan, Pengfei Zhang*, Plasmon-Enhanced Quantum Dot Nanobead-BasedLateral Flow Assay with Lower Background and Improved Sensitivity, ACSApplied Nano Materials, 2025, 8, 4120-4128. 

[13]    Hongyan Zhang, Fei Tan*, Qixia Wang, Peng Xu,Mengyuan Shen, Xiaoping Hu, Heng Wu, Guoliang Zhou, Pengfei Zhang*,Juan Wang*, CRISPR-Cas12a based HSV DNA detection method using quantumdot-labeled immunochromatographic strips, Microchemical Journal, 2024, 207, 112117.doi: 10.1016/j.miroc.2024.112117 发表日期2024-11-4

[12]    Xinyue Tang#, Wenwen Xia#, Huanxing Han,Yucheng Wang, Bolong Wang*, Shouhong Gao*, Pengfei Zhang*,Dual-Fluorescent Quantum Dot Nanobead-Based Lateral Flow Immunoassay forSimultaneous Detection of C-Reactive Protein and Procalcitonin, ACS Applied Bio Materials, 2024, 发表日期2024-10-31, doi: 10.1021/acsabm.4c01230

[11]    Lingzhi Fan, Yue Luo, Wannian Yan, HuanxingHan, Pengfei Zhang*, Fluorescent Lateral Flow Immunoassay Basedon Quantum Dots Nanobeads. Journal of Visualized Experiments, 2024, 208, e67000. doi:10.3791/67000. 发表日期2024-6-28

[10]    Lingzhi Fan, Wannian Yan, Qilong Chen, Fei Tan,Yijie Tang, Huanxing Han, Rujia Yu, Ni Xie, Shouhong Gao, Wansheng Chen*,Zhongjian Chen*, Pengfei Zhang*, One-Component Dual-ReadoutAggregation-Induced Emission Nanobeads for Qualitative and QuantitativeDetection of C-Reactive Protein at the Point of Care, Anal. Chem. 2024, 96, 1, 401–408. 发表日期2023-12-22 doi:10.1021/acs.analchem.3c04441.

[9]    Nanhui Wu, Yijie Cai, Fei Wu, Yulin Liang,Shuyi Liu, Pengfei Zhang, Yeqiang Liu*, C3d ImmunohistochemicalStaining on Paraffin-Embedded Tissue for Diagnosis of Pemphigus, Arch Pathol Lab Med. 2023 Dec 27. doi: 10.5858/arpa.2023-0274-OA. 

[8]    Xiaoqin Zhong, Qiaoting Fu, Yaoqun Wang, LanLong, Wencheng Jiang, Meiyu Chen, Hui Xia, Pengfei Zhang*, FeiTan*, CRISPR-based quantum dot nanobead lateral flow assay for facile detectionof varicella-zoster virus, Applied Microbiology and Biotechnology, 2023,107, 3319–3328 发表日期：13 April 2023 doi: 10.1007/s00253-023-12509-0

[7]    Hui Xia, Xiao Hui Mo, Lian Juan Yang, HongYang, Jing Wen Tan, Qian Yu, Peng Fei Zhang, Fei Tan, Comparisonof samples of blister fluid and scales in the diagnosis of dermatomycosis, Mycoses.2022, 65 (10):969-975. doi: 10.1111/myc.13494.

[6]    Lingzhi Fan#, Jing Yang#, Jianbing Wu#, FugangLi, Wannian Yan, Fei Tan, Madeline Zhang, Mohamed S. Draz*, Huanxing Han*, Pengfei Zhang*, Deeply-dyednanobead system for rapid lateral flow assay testing of drugs at point-of-care,Sensors and Actuators B: Chemical, 2022,362, 131829.  1 July 2022 doi.org/10.1016/j.snb.2022.131829

[5]    Liangwen Hao, Weitao Yang, Yan Xu, TianmingCui, Guoqi Zhu, Weiwei Zeng, Kexin Bian, Hongying Liang, Pengfei Zhang, Bingbo Zhang*, Engineering light-initiatedafterglow lateral flow immunoassay for infectious disease diagnostics, Biosensors and Bioelectronics, 2022, 212, 114411.

[4]  Pengfei Zhang#, Mohamed S. Draz#, Anwen Xiong#, Wannian Yan, Huanxing Han*, Wansheng Chen*, Immunoengineered magnetic-quantum dot nanobead system for the isolation anddetection of circulating tumor cells, Journalof Nanobiotechnology, 2021, 19, 116. 日期2021 Apr 23，doi:10.1186/s12951-021-00860-1

[3] Yuye Wu, Dheerendranath Battalapalli, Mohammed J. Hakeem, Venkatarao Selamneni, Pengfei Zhang*, Mohamed S.Draz*, Zhi Ruan*, Engineered CRISPR-Cas systems for the detection and controlof antibiotic-resistant infections, Journal of Nanobiotechnology, 2021, 19, 401. 2021 Dec 4 DOI:10.1186/s12951-021-01132-8

[2]  Hangfei Chen, Dheerendranath Battalapalli, Mohamed S Draz*, Pengfei Zhang*, Zhi Ruan*, The Application of Cell-Penetrating-Peptides in Antibacterial Agents, Current Medicinal Chemistry, 2021, 28(29), 5896-5925.

[1]  Wannian Yan#; Lingzhi Fan#; Jin Li#; Yijiang Wang;Huanxing Han; Fei Tan*; Pengfei Zhang*; Bimodal size distribution immuno-quantum dots for fluorescentwestern blotting assay with high sensitivity and extended dynamic range, Microchimica Acta, 2020, 187, 598.

8.  一种多重指标数字检测样本的系统、方法及应用，申请号：2025104187138，申请日期：2025年4月3日

7.  一种基于数字层析和CRISPR系统的无扩增超灵敏核酸检测系统及检测方法，申请号：CN202411101442.5，申请日期：2024.8.12

6.  基于RAA结合CRISPR cas12a检测单纯疱疹病毒的试剂盒及检测方法和检测亚型的方法, 申请号：CN2024108027877，申请日期：2024.6.20

5.  一种金属增强量子点荧光纳米球及其制备方法，发明专利，申请号：CN202311098600.1，申请日：2023.8.29.

4.  一种多模式聚集诱导荧光免疫层析试纸及其制备方法，发明专利，申请号：CN202210285423.7，申请日：2022-03-23.

3.  一种彩色乳胶颗粒、复合物、免疫层析试纸及其制备方法，发明专利，申请号：CN202110766151.8，申请日：2021-7-7.授权日：2024-8-28

2.  ULTRA-SENSITIVE DIGITAL RAPID CHROMATOGRAPHICDETECTION SYSTEM AND METHOD FOR ANALYTE,  PCT/CN2021/112311,2021-8-12.

1.  超灵敏数字层析快速检测分析物的系统及方法，发明专利，授权号：ZL 2020108603286,申请日：2020-08-25.授权日期：2024-2-9