一、团队概况（About the Lab）

中文简介： 本研究团队依托同济大学附属东方医院，聚焦骨再生材料学、骨质疏松与骨老化机制、免疫相关疾病分子靶点、衰老相关肌肉萎缩的机制及牙科种植机器人系统的交叉研究。团队融合生物学、材料学、医学与工程学的多学科优势，围绕重大代谢性与退行性疾病的发生机制与转化应用展开系统研究。 研究内容涵盖从生物材料抗氧化机制到细胞代谢与信号通路调控，再到临床口腔智能装备的开发与验证，旨在通过多组学、生物信息学分析与动物模型实验等手段，揭示组织损伤与再生过程中的关键分子网络，推动材料创新与精准医学的临床转化。

英文简介： The research team, based in the Tongji University Affiliated Oriental Hospital, focuses on interdisciplinary studies in bone regeneration materials, mechanisms of osteoporosis and bone aging, immune-related therapeutic targets, aging-related muscle atrophy, and dental implant robotic systems. By integrating biology, materials science, medicine, and engineering, the team conducts systematic research on the molecular and translational mechanisms underlying major metabolic and degenerative diseases. Through multi-omics analysis, bioinformatics, and animal model experiments, we aim to elucidate key molecular networks involved in tissue injury and regeneration, driving innovation in biomaterials and advancing translational and precision medicine.

二、   主要研究方向（Research Focus）

1.骨再生相关材料科学 —— 探索HA/β-TCP双相磷酸钙材料及其抗氧化机制，研发具生物活性与抗应激性能的骨修复支架；

2.骨质疏松与骨老化机制研究 —— 揭示去琥珀酰化酶SIRT5及PRMT6-SIRT5信号轴在骨衰老中的分子调控作用；

3.舍格伦综合征治疗靶点研究 —— 解析LFA-1介导的T细胞浸润与免疫反应机制，为自身免疫性疾病提供新型治疗策略；

4.衰老相关肌肉萎缩机制研究 —— 研究IL-23R在炎症性衰老（inflammaging）及肌肉退行性变化中的作用；

5.牙科种植机器人系统研究 —— 构建基于力反馈与骨密度感应的智能钻孔控制算法，提升精准种植与个体化治疗水平。

6.脂肪组织免疫微环境调控与能量代谢机制研究 —— Panx1介导巨噬细胞极化调控脂肪褐变的机制研究

1.Biomaterials for Bone Regeneration – Investigating HA/β-TCP biphasic calcium phosphate materials and their antioxidant mechanisms to develop bioactive, stress-resistant scaffolds for bone repair.

2.Mechanisms of Bone Aging and Osteoporosis – Elucidating the molecular roles of the desuccinylase SIRT5 and the PRMT6–SIRT5 signaling axis in bone aging and metabolic bone disorders.

3.Therapeutic Targets in Sjögren’s Syndrome – Exploring LFA-1–mediated T-cell infiltration and immune regulation to identify potential therapeutic targets for autoimmune diseases.

4.Mechanisms of Age-Related Muscle Atrophy – Studying IL-23R–driven inflammaging and its contribution to muscle degeneration and chronic inflammation.

5.Dental Implant Robotic Systems – Developing force-feedback–based intelligent drilling algorithms to enhance precision and adaptability in dental implant surgery.

三、科研成果与合作（Publications & Collaborations）

代表性成果：

1.Deng, K., Liu, Z., Dou, W., Cai, Q., Ma, W., & Wang, S. (2022). HA/β-TCP Biphasic Calcium Phosphate Ceramics Derived From Butterfish Bones Loaded With Bone Marrow Mesenchymal Stem Cells Promote Osteogenesis. Frontiers in Materials, 9, 928075. https://doi.org/10.3389/fmats.2022.928075

2.Li, J., Wang, S., Tang, X., Que, L., Han, W., & Yu, B. (2023). Primary maxillary sinus carcinosarcoma with multidisciplinary management: A case report with 4 years follow-up and literature review. BMC Oral Health, 23, 96. https://doi.org/10.1186/s12903-022-02604-5

3.Zhu, Q., Chen, T., Xia, J., Jiang, D., Wang, S., & Zhang, Y. (2022). Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes. Journal of Biomaterials Applications. https://doi.org/10.1177_08853282221111969

4.Mi, W., Guo, H., Yu, W., Wang, S., Pan, T., & Wang, S. (2023). Need for dental care among medical staff working in the China Antarctic stations. International Journal of Circumpolar Health, 82(1), 2179453. https://doi.org/10.1080/22423982.2023.2179453

5.Wang, S., & Pan, W. (2023). Sex-specific gene expression patterns in head and neck squamous cell carcinomas. Heliyon, 9(4), e14890. https://doi.org/10.1016/j.heliyon.2023.e14890

6.Zhong, Q., Cao, X., Shen, Y., Song, Y., Wu, Y., Qu, F., Wang, S., & Xu, C. (2023). Finite element analysis of maxillary first molar with mesial-occlusal-distal-palatal defect restored with different post-and-core strategies. Heliyon, 9(7), e18131. https://doi.org/10.1016/j.heliyon.2023.e18131

7.Wang, Y., Cao, X., Shen, Y., Zhong, Q., Huang, Y., Zhang, Y., Wang, S., & Xu, C. (2023). Initial Development of an Immediate Implantation Model in Rats and Assessing the Prognostic Impact of Periodontitis on Immediate Implantation. Bioengineering, 10(8), 896. https://doi.org/10.3390/bioengineering10080896

8.Li, X., Fang, S., Wang, S., Xie, Y., Xia, Y., Wang, P., Hao, Z., Xu, S., & Zhang, Y. (2024). Hypoxia preconditioning of adipose stem cell-derived exosomes loaded in gelatin methacryloyl (GelMA) promote type H angiogenesis and osteoporotic fracture repair. Journal of Nanobiotechnology, 22, 112. https://doi.org/10.1186/s12951-024-02342-6

9.Yue, G., Huang, W., Weng, B., & Wang, S. (2024). Axial and tilted implant surgical technique assisted by an autonomous dental implant robot: A clinical report. The Journal of Prosthetic Dentistry. https://doi.org/10.1016/j.prosdent.2024.03.046

10.Xi, S., Hu, J., Yue, G., & Wang, S. (2024). Accuracy of an autonomous dental implant robotic system in placing tilted implants for edentulous arches. The Journal of Prosthetic Dentistry. https://doi.org/10.1016/j.prosdent.2024.07.032

11.Song, X., Okabe, K., Ohta, Y., Ohara, G., Toyama, N., Chang, Q., Wang, Y., & Hibi, H. (2023). Family with sequence similarity 20 member B regulates osteogenic differentiation of bone marrow mesenchymal stem cells on titanium surfaces. Acta Biomaterialia, 161, 298-308. https://doi.org/10.1016/j.actbio.2023.02.035

12.Ohara, G., Okabe, K., Toyama, N., Ohta, Y., Xinman, S., Ichimura, N., Sato, K., Urata, Y., & Hibi, H. (2023). Hyperthermia maintains death receptor expression and promotes TRAIL-induced apoptosis. Journal of Oral Pathology & Medicine, 52(8), 718-726. https://doi.org/10.1111/jop.13457

13.Ohara, G., Okabe, K., Toyama, N., Ohta, Y., Song, X., Tsuboi, M., Kouyama, N., Sato, K., & Hibi, H. (2024). Stem cell therapy based on human periodontal ligament stem cells preactivated with TNF-α as for human head and neck squamous cell carcinoma. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 36(2), 217-226. https://doi.org/10.1016/j.ajoms.2023.08.006

14.Xu Q, Yao Z, Yu X, Ying J, Zhu J, Lu L, Hu Z, Jiang H, Wang H. A scoring-based clinical grading model for xanthelasma palpebrarum: predicting treatment frequency and prognosis. Arch Dermatol Res. 2024 Sep 12;316(8):614. doi: 10.1007/s00403-024-03298-1. PMID: 39266881.

15.Yang HW, Xu QH, Li QY, Zhang LJ, Shu HY, Liang RB, Pan YC, Shao Y. Abnormal fractional amplitude of low-frequency fluctuations in MOG-lgG optic neuritis patients: a resting-state functional MRI study. J Integr Neurosci. 2022 Mar 23;21(2):60. doi: 10.31083/j.jin2102060. PMID: 35364648.

16.Gui, Q., Ding, N., Liu, J., Zhao, Y., Du, A., Zhu, J., Wu, H., Wu, M., Wang, Y., & Zhu, L. (2024). MiR-200a-3p in Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Attenuates UVB-Induced Skin Inflammatory Response and Oxidative Stress via Keap1-Nrf2 Pathway. Stem Cells International, 2025(1), 7831890. https://doi.org/10.1155/sci/7831890

17.Gui, Q., Ding, N., Wu, H., Liu, J., Geng, Y., Zhu, J., Gao, M., Du, A., Yue, B., & Zhu, L. (2025). Development of a pH-Responsive Antimicrobial and Potent Antioxidant Hydrogel for Accelerated Wound Healing: A Game Changer in Drug Delivery. Advanced Biology, 9(2), 2400358. https://doi.org/10.1002/adbi.202400358

18.Gui, Q., Ding, N., Yao, Z., Wu, M., Fu, R., Wang, Y., Zhao, Y., & Zhu, L. (2024). Extracellular vesicles derived from mesenchymal stem cells: The wine in Hebe's hands to treat skin aging. Precision Clinical Medicine, 7(1), pbae004. https://doi.org/10.1093/pcmedi/pbae004

19.Ding, N., Fu, X., Gui, Q., Wu, M., Niu, Z., Du, A., Liu, J., Wu, H., Wang, Y., Yue, X., & Zhu, L. (2024). Biomimetic Structure Hydrogel Loaded with Long-Term Storage Platelet-Rich Plasma in Diabetic Wound Repair. Advanced Healthcare Materials, 13(10), 2303192. https://doi.org/10.1002/adhm.202303192

20.Gui, Q., Luo, W., Wu, D., Liu, J., Zhou, D., Zhu, X., Wu, M., & Zhu, L. (2025). Construction and validation of a lipid metabolism-related genes prognostic signature for skin cutaneous melanoma. Biochemical and Biophysical Research Communications, 775, 152115. https://doi.org/10.1016/j.bbrc.2025.152115

合作单位：同济大学附属东方医院

团队成员（Team Members）

·       王少海 教授-负责人 / Principal Investigator

·       宋昕蔓 — 博士后 / Postdoctoral Fellows

·       徐千惠、桂启翔—博士研究生 / PhD Candidates

·       陈丽菲、杜兆涵、梁爽— 硕士研究生 / Master Students

项目情况（Research Projects）

一、近五年主持或参加的国家自然科学基金项目/课题：

1. 国家自然科学基金委员会，面上项目（No. 81870808）： 鱼骨来源BCP对激活CaSR调控整合素β1（ITGB1）表达和功能的机制研究， 项目周期：2019-01-01 至 2022-12-31，资助经费：57万元，资助期满，主持。

二、近五年主持或参加的其他科研项目/课题（国家自然科学基金项目以外）：

1. 上海申康医院发展中心，重大临床研究项目（No. SHDC2020CR2042B）： 第二轮《促进市级医院临床技能与临床创新三年行动计划（2020–2022）》重大临床研究项目， 项目周期：2020-01 至 2023-01，资助经费：38.4万元，在研，参与。
2. 上海市浦东新区卫生系统特色专病建设资助项目（No. PWZzb2022-17）： 项目名称：特色专病建设项目， 项目周期：2022年起，资助经费：20万元，在研，主持。
3. 上海市东方医院（同济大学附属东方医院）创新与转化促进项目（2025）： 项目名称：《一种手持便携式口腔数字观测设备的改进研发》， 项目周期：2025年，资助经费：10万元，在研，主持。

Recent Research Projects (Past Five Years)

1. National Natural Science Foundation of China (NSFC) – General Program (No. 81870808): Regulatory mechanisms of fish bone–derived BCP on CaSR-mediated ITGB1 expression and function, Funding Period: Jan 2019 – Dec 2022, Funding: ¥570,000, Completed (PI).

2. Shanghai ShenKang Hospital Development Center – Major Clinical Research Project (No. SHDC2020CR2042B): “Three-Year Action Plan for Promoting Clinical Innovation in Municipal Hospitals (2020–2022)”, Funding Period: Jan 2020 – Jan 2023, Funding: ¥384,000, Ongoing (Co-investigator).

3. Specialty Feature Construction Project of Shanghai Pudong New Area Health Commission (Grant No. PWZzb2022-17): Funding: ¥200,000, Ongoing (PI).

4. Innovation and Translational Promotion Project, Shanghai Oriental Hospital (Tongji University Affiliated Oriental Hospital, 2025): Improved development of a handheld portable oral digital observation device, Funding: ¥100,000, Ongoing (PI).

科研方向与在研课题简介

（一）骨再生相关材料科学（Biomaterials for Bone Regeneration）

研究基于双相磷酸钙（HA/β-TCP）材料的骨修复与抗氧化机制，探索其在骨质疏松与骨缺损再生中的应用潜力。 团队发现，传统单相羟基磷灰石（HA）材料因产生活性氧（ROS）可引发氧化应激并导致成骨细胞凋亡，而鱼骨来源双相FB-BCP材料能够显著减少ROS水平并保护成骨细胞活性。研究进一步探讨该材料通过调控NADPH氧化酶（NOXs）及Ca²⁺信号通路抑制凋亡的分子机制，为抗氧化功能型生物材料的开发提供实验依据。

English summary: This research focuses on HA/β-TCP biphasic calcium phosphate biomaterials and their antioxidant functions in bone regeneration. Compared with traditional single-phase HA, the fish bone–derived FB-BCP material significantly reduces ROS generation and protects osteoblast viability. The study explores how the biphasic structure modulates NADPH oxidase (NOX) activity and Ca²⁺-mediated apoptotic signaling, providing theoretical support for the design of bioactive and antioxidant bone scaffolds.

（二）骨质疏松与骨老化机制研究（Mechanisms of Bone Aging and Osteoporosis）

从分子层面解析去琥珀酰化酶SIRT5在成骨细胞代谢、分化与DNA损伤修复中的作用。 团队发现，PRMT6–SIRT5信号轴在衰老骨组织中失调，导致能量代谢紊乱及蛋白琥珀酰化水平异常升高，进而激活DNA损伤通路（γH2AX、P21），加速骨老化与骨质流失。通过琥珀酰化组学与细胞功能实验揭示“PRMT6–SIRT5–琥珀酰化–DNA损伤”调控轴，为代谢性骨病的干预提供新理论依据。

English summary: This direction investigates the PRMT6–SIRT5 signaling axis in bone aging and osteoporosis. The team revealed that SIRT5 downregulation in aged osteoblasts leads to enhanced protein succinylation, disrupted energy metabolism, and activation of DNA damage response pathways (γH2AX, P21). These findings establish the PRMT6–SIRT5–succinylation–DNA damage regulatory axis as a key mechanism in skeletal aging and a potential target for metabolic bone disease therapy.

（三）舍格伦综合征治疗靶点研究（Therapeutic Targets in Sjögren’s Syndrome）

通过生物信息学分析与实验验证，研究淋巴细胞功能相关抗原-1（LFA-1）在舍格伦综合征（Sjögren’s Syndrome, SS）中的作用机制。 实验结果表明，LFA-1在SS患者唇腺组织及CD4⁺T细胞中高表达，促进淋巴细胞浸润及局部炎症反应。动物模型与细胞实验进一步证实LFA-1抑制剂可显著降低炎症浸润水平，改善腺体功能，为SS免疫治疗提供新靶点。

English summary: This project investigates the role of LFA-1 (Lymphocyte Function-Associated Antigen-1) in Sjögren’s Syndrome. LFA-1 is highly expressed in the salivary glands and CD4⁺ T cells of SS patients, enhancing lymphocytic infiltration and local inflammation. Experimental and animal studies demonstrated that LFA-1 inhibition alleviates glandular damage, suggesting its potential as a therapeutic target for autoimmune diseases.

（四）衰老相关肌肉萎缩机制研究（Mechanisms of Age-Related Muscle Atrophy）

结合遗传流行病学与实验验证，探讨白介素-23受体（IL-23R）在衰老相关性肌肉退行性变化中的作用。 通过孟德尔随机化（Mendelian Randomization）分析，发现IL-23R与肌肉萎缩存在潜在因果关联，其促进IL-17、IL-22等促炎细胞因子分泌，削弱组织修复能力并加剧炎症性衰老（inflammaging）。研究结果为肌肉老化防治提供分子靶点依据。

English summary: This research explores the causal role of Interleukin-23 receptor (IL-23R) in age-related muscle atrophy using Mendelian randomization and experimental verification. IL-23R promotes pro-inflammatory cytokines (IL-17, IL-22) and impairs tissue repair, contributing to chronic inflammation and muscle degeneration during aging.

（五）牙科种植机器人系统（Dental Implant Robotic System）

开发基于力反馈与骨密度感应的种植机器人控制算法，建立骨内力反馈与钻孔参数的量化模型。 研究通过冰鲜下颌骨实验结合红外定位与六轴力/扭矩传感技术，发现钻孔速度、深度与直径等参数与力反馈值呈强线性相关（R²=0.998）。研究成果为智能种植机器人自主控制算法提供生物力学数据支撑，助力临床精准种植应用。

English summary: This study establishes a quantitative correlation between drilling parameters and intraosseous force feedback in a robot-assisted dental implant system. Using a 6-axis force/torque sensor and infrared tracking, strong linear relationships (R²=0.998) were identified between drilling depth, speed, and diameter. The findings provide critical biomechanical data for developing autonomous control algorithms in dental implant robotics.

（六）脂肪组织免疫代谢机制研究（Immunometabolic Regulation in Adipose Tissue） 研究聚焦Panx1介导的巨噬细胞极化对脂肪褐变的调控机制。肥胖及相关代谢综合征的核心病理基础是白色脂肪组织异常扩张和功能障碍。研究发现，肥胖人群皮下脂肪组织中Panx1表达显著升高，其介导ATP等代谢物外流，促使脂肪相关巨噬细胞（ATMs）向促炎性M1表型极化，改变细胞因子分泌谱，从而抑制脂肪源性干细胞（ASCs）向产热性米色脂肪分化，导致脂肪功能失衡与代谢紊乱。本研究拟进一步阐明Panx1调控巨噬细胞极化及ASCs分化的分子机制，为改善肥胖及相关代谢性疾病提供潜在的新策略。

English summary: This study focuses on the regulatory mechanism by which Panx1-mediated macrophage polarization influences adipose browning. Obesity and related metabolic disorders are characterized by abnormal expansion and dysfunction of white adipose tissue. Elevated Panx1 expression in subcutaneous fat of obese individuals promotes ATP efflux, drives adipose tissue macrophages (ATMs) toward the pro-inflammatory M1 phenotype, and alters cytokine profiles, thereby suppressing adipose stem cell (ASC) differentiation into thermogenic beige adipocytes. This work aims to elucidate the molecular pathways of Panx1 in modulating macrophage polarization and ASC differentiation, providing new insights for therapeutic strategies against obesity and metabolic diseases.

 2022年上海市浦东新区卫健委 特色专病 牙列缺损种植修复，第一申请人。项目编号：PWZzb2022-17，20万元

 2020年上海申康医院发展中心第二轮《促进市级医院临床技能与临床创新三年行动计划（2020-2022年）》重大临床研究项目240万元。担任乙方分中心负责人38.4万元. 基金号SHDC2020CR2042B

2019.01-2022.12国家自然科学基金“鱼骨来源BCP对激活CaSR调控整合素β1（ITGB1）表达和功能的机制研究”第一申请人。项目批准号：81870808,资助基金57万元。

 2013.01-2016.12获得国家自然科学基金《仿生修复材料透光率数学模型建立与色知觉的相关性研究》项目批准号：81271177, 第一申请人。资助基金65万元。

2011-01-01至2013-11-30获得上海市科学技术委员会医学引导基金《天然牙冠透光率数学模型建立及其分级的研究》基金号114119a3600，第一申请人，资助金额10万元

 2009年获得国家自然科学基金《联合应用单磷酸酰脂质的WapA防龋DNA疫苗增强粘膜免疫反应的效应及其机制研究》基金号：30901679第二申请人。资助金额34万元

 2006年获国家自然科学基金《中国汉族健康成人牙龈及牙槽粘膜颜色测量分析及比色系统的建立》基金号30672344，第三申请人， 资助金额28万元

Initial Development of an Immediate Implantation Model in Rats and Assessing the Prognostic Impact of Periodontitis on Immediate Implantation,Bioengineering,2023,10(8)2-18

Finite element analysis of maxillary first molar with mesial-occlusal-distal-palatal defect restored with different post-and-core strategies . 2023

Sex-specific gene expression patterns in head and neck squamous cell carcinomas . 2023  

Preparation and characterization of two novel    osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes.Journal of Biomaterials Applications [J].2022

Primary maxillary sinus carcinosarcoma with multidisciplinary management: A case report with 4 years follow-up and literature review,BMC Oral Health,2022,10(7)：51-58

HA/β-TCP Biphasic Calcium Phosphate Ceramics Derived From Butterfish Bones Loaded With Bone Marrow Mesenchymal Stem Cells Promote Osteogenesis，Frontiers in Materials,Received: 28 April 2022,Accepted: 17 June 2022

Preparation and characterization of porous HA/beta-TCP biphasic calcium phosphate derived from butterfish bone, Materials Technology, 2021, 0(0)：1-8. SCIE.  

Design and construction of a microporous CO3 2
-containing HA/βTCP biphasic ceramic as a novel bone graft material. Materials Research Express. 7 (2020) 025401

Extraction and Characterization of HA/ß-TCP Biphasic Calcium Phosphate from Marine Fish. Materials Letters 236（2019）680-682

The preparation and characterization of HA/β-TCP biphasic ceramics from fish bones. Ceramics International, 2017,43 (15) :12213–12220

获得总后勤部军队医疗成果三等奖《外伤致前牙缺损变色后色彩复现的相关性研究》，证书号：2009－3－251－1  第一完成人 

陕西省科学技术进步二等奖  牙齿纵折的保存修复  证书号:2000-375

荣获2018上海白玉兰口腔优秀病例奖。二等奖。第一完成人。

专利

王少海; 邓可晗; 朱庆丰; 陈海文; 窦文雪; 李静; 马威; 蔡齐, 鲳鱼骨来源双相磷酸钙多孔生物陶瓷骨支架材料及其制备方法与应用, 2022-06-24, 中国, CN202210721336.1. 

王少海一种具有高温预警作用的牙科钻头2017.12.22,中国,专利号：ZL 201621373799X   申请时间2016.12.14

 王少海一种牙科种植印模定位延长装置2017.12.22,中国,专利号：ZL 201621391607.8申请时间2016.12.19

 王少海 一种提示温度变化的琼脂印模输送器2017.12.22,中国,专利号：ZL201621391606.3 申请时间2016.12.19

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