副研究员 

施晓疌

博士后

林哲伊

硕博连读研究生

华南农业大学  学士

在读研究生

杭成文

博士研究生

北京大学 硕士

扬州大学 学士

卢仁宏

硕博连读研究生

同济大学 学士

刘军阳

硕博连读研究生

烟台大学大学 学士

李怡美

博士研究生

东北农业大学 硕士

沈阳农业大学 学士

张明帅

硕士研究生

新乡医学院 学士

付一荻

硕士研究生

锦州医科大学 学士

本科实习生

王思雨

锦州医科大学

我们团队的项目主要为：

一、多能干细胞状态的转换和体细胞重编程的分子机制

1. 聚焦重要的转录因子SALL家族在多能干细胞干性维持、状态转换和三胚层分化过程中的重要作用和分子机制。

2. 运用CRISPR/Cas系统筛选与体细胞重编程和多能干细胞分化相关的新基因并研究其机制。

二、基于多能干细胞的心脏生物起搏和再生治疗，探究调控心脏发育的分子机制，以阐明心脏疾病的发病机制及新的治疗靶点的研发

1. 通过建立高效的心脏起搏细胞分化方法获得功能性的起搏细胞为生物起搏器的研发提供细胞来源。

2. 通过诱导多能干细胞分化为心房肌细胞，在体外建立房颤的模型（2D, 3D），探索应用新型纳米材料进行房颤的实验性治疗。

3. 研究参与早期心脏发育的重要转录因子的调控机制，通过干细胞来源的心肌细胞建立心脏疾病的体外模型，研发干预的新靶点。

国家自然科学基金（面上项目）：Sall1调控多能干细胞naïve和primed状态转化的机制研究 主持

国家自然科学基金（基础科学中心项目）：重大心脏病的起源和干预骨干

国家自然科学基金（重点项目）：蛋白激酶介导的心肌细胞增殖在心肌损伤修复中的作用及机制研究骨干

中国医学科学院：心律的起源与调控创新单元 骨干

科技部“一带一路”创新人才交流外国专家项目：纳米生物材料在干细胞治疗心脏疾病中的安全、有效性研究负责人

上海市前沿科学研究基地：上海市催化医学前沿科学研究基地 骨干

发表论文

1. Chengwen Hang, Huixin Guo,Mohamed S Moawad, Christie Sayes, Yi-Han Chen, Jian Yang. Application ofCerium Oxide Nanoenzymes (CeONZs) in Human Pluripotent Stem Cell-DerivedCardiomyocytes. Methods in Molecular Biology, Co-correspondingauthor, 2025, Mar 20. Doi:10.1007/7651_2025_606.

2. Zhaoru Ma, Shuping Tan,Renhong Lu, Peixin Chen, Yukun Hu, Tenghui Yang, Hao Wu, Zhexin Zhu, Jiayi Guo,Xi Chen, Jian Yang, Wensheng Zhang, Ying Ye.Interplay of chromatin remodeling BAF complexes in mouse embryonic and epiblaststem cell conversion and maintenance. Journal of BiologicalChemistry, Co-corresponding author, 2024, Dec 25:108140. Doi:10.1016/j.jbc.2024.108140.

3. Renhong Lu, Bowen Lin, ZheyiLin, Hui Xiong, Junyang Liu, Li Li, Zheng Gong, Siyu Wang, Mingshuai Zhang, JieDing, Chengwen Hang, Huixin Guo, Duanyang Xie, Yi Liu, Dan Shi, Dandan Liang,Zhen Liu, Jian Yang, Yi-Han Chen. Hypo-osmolaritypromotes naïve pluripotency by reshaping cytoskeleton and increasing chromatinaccessibility. Journal of Advanced Research, Co-corresponding author, 2024, Dec 4:S20901232(24)00556-3.Doi: 10.1016/j.jare.2024.11.037.

4. Hui Xiong, Bowen Lin,Junyang Liu, Renhong Lu, Zheyi Lin, Chengwen Hang, Wenjun Liu, Lei Zhang, JieDing, Huixin Guo, Mingshuai Zhang, Siyu Wang, Zheng Gong, Duanyang Xie, Yi Liu,Dan Shi, Dandan Liang, Zhen Liu, Yi-Han Chen, Jian Yang. Sall2 regulatesneural differentiation of mouse embryonic stem cells through Tuba1a. CellDeath and Disease, Co-corresponding author, 2024 Sep 30, 15(9): 710. Doi:10.1038/s41418-024-07088-5.

5. Zheyi Lin, Bowen Lin,Chengwen Hang, Renhong Lu, Hui Xiong, Junyang Liu, Siyu Wang, Zheng Gong,Mingshuai Zhang, Desheng Li, Guojian Fang, Jie Ding, Xuling Su, Huixin Guo, DanShi, Duanyang Xie, Yi Liu, Dandan Liang, Jian Yang, Yi-Han Chen. Anew paradigm for generating cardiac pacemaker cells from mouse pluripotent stemcells. Signal Transduction and Targeted Therapy, Co-correspondingauthor, 2024. 9(10): 4564-4578. Doi: 10.1038/s41392-024-01942-w.

6. Chengwen Hang, Mohamed S Moawad,Zheyi Lin, Huixin Guo, Mingshuai Zhang, Renhong Lu, Junnyang Liu, Dan Shi,Duanyang Xie, Yi Liu, Dandan Liang, Yi-Han Chen, Jian Yang. Biosafe cerium oxidenanoenzymes protect human pluripotent stem cells and cardiomyocytes from oxidativestress. Journal of Nanobiotechnology, Co-correspondingauthor, 2024. 22(1): 132. Doi: 10.1186/s12951-024-02383-x.

7. Huixing Guo, Chengwen Hang, BowenLin, Zheyi Lin, Hui Xiong, Mingshuai Zhang, Rehong Lu, Junyang Liu, Dan Shi, DuanyangXie, Yi Liu, Dandan Liang, Jian Yang, Yi-Han Chen. HAND factorsregulate cardiac lineage commitment and differentiation from human pluripotentstem cells. Stem Cell Research and Therapy, Co-correspondingauthor, 2024. 15(1): 31. Doi: 10.1186/s13287-024-03649-9.

8. Duanyang Xie, Ke Xiong, NianguoDong, Guanghua Wang, Qicheng Zou, Beihua Shao, Zhiwen Chen, Luxin Wang, YuKong, Xu Wang, Xuling Su, Wenli Bai, Jian Yang, Yi Liu, Bin Zhou,Yi-Han Chen. An endogenous cholinergic system controls electrical conduction inthe heart. European Heart Journal, 2024. Oct 22:ehae699. Doi:10.1093/eurheartj/ehae699.

9. Fulei Zhang, Huixing Zhou, JinfenngXue, Yuemei Zhang, Liping Zhou, Junwei Leng, Guojian Fang, Yuanyuan Liu, YanWang, Hongyu Liu, Yahan Wu, Lingbin Qi, Ruan Duan, Xiaoyu He, Yan Wang, Yi Liu,Li Li, Jian Yang, Dandan Liang, Yi-Han Chen.Deficiency of transcription factor SP1 contributes to hypertrophic cardiomyopathy.Circulation Research, 2024. 134(3):290-306. Doi:10.1161/CIRCREESAHA.123.323272.

10. Le Ni, Bowen Lin, Yanping Zhang,Lingjie Hu, Jianghua Lin, Fengmei Fu, Meiting Shen, Can Li, Lei Chen, JianYang, Dan Shi, Yi-Han Chen. Histone modification landscape and thesignificance of H3K27me3 in myocardial ischaemia/reperfusion injury. ScienceChina Life Sciences, 2023. Jun, 66(6):1264-1279. Doi:10.1007/s11427-022-2257-9.

11. Le Ni, Bowen Lin, Meiting Shen, Can Li, Lingjie Hu,Fengmei Fu, Lei Chen, JianYang, Dan Shi. PKM2 deficiencyexacerbates gram-negative sepsis-induced cardiomyopathy via disrupting cardiaccalcium homeostasis. Cell Death Discovery, 2022. Dec 23,8(1): 496. Doi: 10.1038/s41420-022-01287-9. 

12. Le Ni, Bowen Lin, LingjieHu, Ruoyu Zhang, Fengmei Fu, Meiting Shen, Jian Yang, Dan Shi. Pyruvate kinase M2 protects heart frompressure overload-induced heart failure by phosphorylating RAC1. Journal of the American Heart Association, Co-correspondingauthor, 2022. Jun 7, 11(11):e024854. Doi: 10.1161/JAHA.121.024854 .

13. Jingjing Liu, Xianting Ke, Luxin Wang, Yangyang Zhang, and Jian Yang. Deficiencyof cold-inducible RNA-binding protein exacerbated monocrotaline-induced pulmonaryartery hypertension through Caveolin1 and CAVIN1. Journal of Cellular andMolecular Medicine, Co-corresponding author, online March 23, 2021.25(10): 4732-4743 Doi: 10.1111/jcmm.16437. 

14. Jian Yang,Haifeng Fu, Cheryl Tam and Pentao Liu. Expanded potential: the key to syntheticembryo? Co-corresponding author, Current Opinion in Genetics and Development, 2020, 64: 72-77. 

15. NicholasBredenkamp, JianYang, James Clarke, GiulianoGiuseppe Stirparo, Ferdinand von Meyenn, Sabine Dietmann, Duncan Baker,Rosalind Drummond, Yongming Ren, Chuman Wu, Maria Rostovskaya, Sarah Eminli-Meissner,Austin Smith, and Ge Guo. Wnt inhibition facilitates RNA-mediated reprogrammingof human somatic cells to naïve pluripotency. Stem Cell Reports, Co-first author, 2019, 13(6): 1083-1098. 

16. Jian Yang, Sandeep S. Rajan,Mathias J Friedrich, Guocheng Lan, Xiangang Zou, Hannes Ponsting, Dimitrios AGaryfallos, Pentao Liu, Allan Bradley, and Emmanouil Metzakopian. Genome-scaleCRISPRa screen identifies novel factors for cellular reprogramming. Stem Cell Reports, Co-first author, 2019, 12(4):754-771.

17. Jian Yang, David Ryan, GuochengLan, Xiangang Zou and Pentao Liu. Establishment in vitro of Expanded PotentialStem Cells from mouse preimplantation embryos and embryonic stem cells. Co-corresponding author, Nature Protocols, 2019, 14, 350-378.

18. Jian Yang, David J. Ryan, Wei Wang,Jason Cheuk-Ho Tsang, Guocheng Lan, Hideki Masaki, Xuefei Gao, Liliana Antunes,Yong Yu, Zhexin Zhu, Juexuan Wang, Aleksandra A. Kolodziejczyk, Lia S. Campos,Cui Wang, Fengtang Yang, Zhen Zhong, Beiyuan Fu, Melanie Eckersley-Maslin,Michael Woods, Yosuke Tanaka, Xi Chen, Adam C. Wilkinson, James Bussell, JacquiWhite, Ramiro Ramirez-Solis,Wolf Reik, Berthold Göttgens, Sarah A. Teichmann, Patrick P. L. Tam, HiromitsuNakauchi, Xiangang Zou, Liming Lu and Pentao Liu. Establishment of murineexpanded potential stem cells. Nature, Co-first author, 2017, 550, 393-397.DOI: 10. 1038/nature24052 .

19. Jian Yang, Wei Wang, Jolene Ooi,Lia Campos, Liming Lu and Pentao Liu. Signaling through retinoic acid receptorsis required for reprogramming of both MEFs and EpiSCs to iPSCs. Stem Cells, 2015, 33(5): 1390-1404.

20. Jian Yang, Anouk L. van Oosten, Thorold W. Theunissen, Ge Guo, Jose C.R. Silva and Austin Smith Stat3 Activation Is Limiting forReprogramming to Ground State Pluripotency. Cell Stem Cell, 2010, 7(3):319-328. 

21. Liudan Jiang, Jiaying Yin,Maoxiang Qian, Shaoqin Rong, Shengqi Zhang, Kejing Chen,Chengchen Zhao, Yuanqing Tan, Jiayin Guo, Hao Chen, Siyun Gao, Tingting Liu, YiLiu, Bin Shen, Jian Yang, Yong Zhang, Fei-Long Meng, Jinchua Hu, Honghui Ma,Yi-Han Chen. UdgX-mediated uracil sequencing at single-nucleotide resolution. J Am Chem Soc, 2022, 143(3): 1323-1331. 

22. SiyunGao, Haifeng Sun, Kejing Chen, Xueying Gu, Hongyu Chen, Liudan Jiang, Lei Chen,Shengqi Zhang, Yi Liu, Dan Shi, Dandan Laing, Liang Xu, Jian Yang, Yanjiao Ruan, Hao Chen, Bin Shen, Honghui Ma, Yi-HanChen. Depletion of m6A reader protein YTHDC1 induces dilated cardiomyopathy byabnormal splicing of Titin. Journal of Cellular and Molecular Medicine, online, October 30, 2021. 25(23): 10879-10891 Doi: 10.1111/jcmm.16955. 

23. Ge Guo, Giuliano Giuseppe Stirparo, Stanley E. Strawbridge, DanielSpindlow, Jian Yang, James Clarke, Anish Dattani, Ayaka Yanagida, MengAmy Li, Sam Myers, Buse Nurten Ozel, Jennifer Nichols, and Austin Smith. Humannaïve epiblast cells possess unrestricted lineage potential. Cell Stem Cell,online April 7, 2021.31(9): 951-964 Doi.org/10.1016/j.stem.2021.02.025. 

24.  Duanyang Xie, Ke Xiong, Xuling Su, Guanghua Wang,Qiang Ji, Qicheng Zou, Lingling Wang, Yi Liu, Dandan Liang, Jinfeng Xue, LuxingWang, Xueting Gao, Xingdong Gu, Hongyu Liu, Xiaoyu He, Li Li, Jian Yang, YoumingLu, Luying Peng, Yi-Han Chen. Identification of an endogenous glutamatergic transmittersystem controlling excitability and conductivity of atrial cardiomyocytes. Cell Research, online 06 April 2021. 31(9): 951-964 Doi: 10.1038/s41422-021-00499-5. 

25. Yanping Zhang, Le Ni, BowenLin, Lingjie Hu, Zheyi Lin, Jian Yang, Jinyu Wang, Honghui Ma, Yi Liu, Jian Yang, Jianghua Lin, Liang Xu, Liqun Wu and Dan Shi. SNX17 protects theheart from doxorubin-induced cardiotoxicity by modulating LMOD2 degradation. Pharmacological Research, 2021, 169: 105642. Online, April 30, 2021. Doi: 10.1016/j.phrs.2021.105642. 

26. DandanLiang, Zhigang Xue, Jinfeng Xue, Duanyang Xie, Ke Xiong, Huixing Zhou, FuleiZhang, Xuling Su, Guanghua Wang, Qicheng Zou, Yi Liu, Jian Yang, Honghui Ma, Luying Peng, Chunyu Zeng, Gang Li, Li Wangand Yi-han Chen. Sinoatrial node pacemaker cells share dominant biological propertieswith glutamatergic neurons. Protein& Cell, online, February6, 2021. 12(7): 545-556 Doi.org/10.1007/s13238-020-00820-9. 

27. DandanLiang, Jinfeng Xue, Li Geng, Liping Zhou, Bo Lv, Qiao Zeng, Ke Xiong, HuixingZhou, Duanyang Xie, Fulei Zhang, Jie Liu, Yi Liu, Li Li, Jian Yang, ZigangXue and Yi-Han Cheng. Cellular and molecular landscape of mammalian sinoatrialnode revealed by single-cell RNA sequencing. Nature Communications, 2021, 12: 287. 

28. DuanyangXie, Li Geng, Ke Xiong, Tingting Zhao, Shuo Wang, Jinfeng Xie, Cheng Wang, GuanghuaWang, Zhiqiang Feng, Huixing Zhou, Yini Li, Li Li, Zhigang Xue, Jian Yang, HonghuiMa, Dandan Liang and Yi-Han Chen. Cold-inducible RNA-binding protein prevents anexcessive heart rate response to stress by targeting phosphodiesterase. Circulation Research, 2020, 126: 1706-1720. 

29. Jinyu Wang, Bowen Lin, Yanping Zhang, Le Ni, Lingjie Hu, Jian Yang, Liang Xu,Dan Shi and Yi-Han Cheng. The regulatory role of histone modification on geneexpression on the early stage of myocardial infarction. Frontiers in Cardiovascular Medicine, 2020, 7: 594325. 

30. DuanyangXie, Li Geng, Shuo Wang, Ke Xiong, Tingting Zhao, Guanghua Wang, Zhiqiang Feng,Fei Lv, Cheng Wang, Dandan Liang, Dan Shi, Xiue Ma, Yi Liu, Jian Yang, Chao Zhang and Yi-Han Chen. Cold-inducible RNA-bindingprotein modulates atrial fibrillation onset by targeting multiple ion channels. Heart Rhythm, 2020, Jan 7, pii: S1547-5271(20)30002-3. 

31. Adam C. Wilkinson, David J.Ryan, Iwo Kucinski, Wei Wang, Jian Yang, Sonia Nestorowa, Evangelia Diamanti, JasonCheuk-Ho Tsang, Juexuan Wang, Lia S. Campos, Fengtang Yang, Beiyuan Fu, NicolaWilson, Pentao Liu and Berhold Gottgens. Expanded potential stem cell media asa tool to study human hematopoiesis in vitro. Experimental Hematology,  2019, 76:1-12.e5. 

32. Xuefei Gao,Monika Nowak-Imialek, Xi Chen, Dongsheng Chen, Doris Herrmann, Degong Ruan,Andy Chun Hang Chen, Melanie A. Eckersley-Maslin, Shakil Ahmad, Yin Lau Lee,Toshihiro Kobayashi, David Ryan, Jixing Zhong, Jiacheng Zhu, Jiacheng Zhu, Jian Wu, Guocheng Lan, Stoyan Petkov, JianYang, Liliana Antunes, Lia S. Campos, Beiyuan Fu, Shengpeng Wang, Yu Yong,Xiaoming Wang, Song-Guo Xue, Liangpeng Ge, Zuohua Liu, Yong Huang, Tao Nie,Peng Li, Donghai Wu, DUanqing Pei, Yi Zhang, Liming Lu,  Fengtang Yang, Susan J. Kimber, Wolf Reik,Xiangang Zou, Zhouchun Shang, Liangxue Lai, Azim Surani, Patrick P.L. Tam, Asif Ahmed, William Shu Biu Yeung, SarahA. Teichmann, Heiner Niemann and Pentao Liu. Establishment of porcine and humanexpanded potential stem cells. Nature Cell Biology, 2019, 21, 687-699. 

33. Jindun Zhang, Xuefei Gao, Jian Yang, Xiaoying Fan, Wei Wang, YanfengLiang, Lihong Fan, Hongmei Han, Xiaorong Xu, Fuchou Tang, Siqin Bao, Pentao Liuand Xihe Li. Xist intron 1 repression by TALE designer transcriptional factorimproves somatic cell reprogramming in mice. Stem Cells, 2019, 37(5): 599-608. DOI: 10.1002/stem.2928. 

34. LixiaZhao, Zixin Wang, Jindun Zhang, JianYang, Xuefei Gao, Baojiang Wu, Gaoping Zhao, Siqin Bao, Shuxiang Hu, PentaoLiu and Xihe Li. Characterization of the single-cell derived bovine inducedpluripotent stem cells. Tissue and Cell, 2017, 49: 521-527. 

35. Xuefei Gao, Jian Yang, Jason Tsang, JoleneOoi, Donghai Wu and Pentao Liu. Reprogramming to Pluripotency Using DesignerTALE Transcription Factors Targeting Enhancers. Stem Cell Reports, 2013, 1(2): 183-197.

36. Wei Wang, Jian Yang, Hui Liu, Dong Lu, Xiongfeng Chen , Zenon Zenonos, Lia S. Campos, Roland Rad, Ge Guo, Shujun Zhang, Allan Bradley, and Pentao Liu. Rapid and efficient reprogramming of somaticcells to induced pluripotent stem cells by retinoic acid receptor gamma andliver receptor homolog 1. Proc Natl Acad Sci U.S.A, 2011,108(45): 18283-18288.

37. Ge Guo, Jian Yang, Jennifer Nichols, John Simon Hall, Isobel Eyres,William Mansfield and Austin Smith. Klf4 reverts developmentally programmed restriction of ground state pluripotency. Development, 2009, 136(7): 1063-1069.

38. Mia Buehr, Stephen Meek,Kate Blair, Jian Yang, Janice Ure, Jose Silva, Renee McLay, John Hall, Qi-Long Ying and Austin Smith. Capture of Authentic Embryonic Stem Cells from Rat Blastocysts. Cell, 2008, 135(7): 1287-1298.

专利

1. Cells and methods for obtaining them. Liu Pentao, Wang Wei, Yang Jian. CA 2773291A1; US2012/0315703 A1; SG 178613 A1;CN 102822345A; SG10201405455U A; US 9512407 B2;EP 2475774A1; WO 2011/027180A1.

2. In vitroproduction of expanded potential stem cells. Liu Pentao, Ryan David, Gao Xuefei,Wang Wei, Yang Jian. WO 2016/079146A1; US 10745760 B2